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Rose-Burek-Caturegli Laboratory

 

       
Noel R. Rose, M.D., Ph.D.
C. Lynne Burek, Ph.D.
Patrizio Caturegli, M.D.

 

Our laboratory is dedicated to the study of autoimmune diseases. It includes both a research component and a clinical component.

Research Laboratory
Our research laboratory focuses on the study of two organ-specific autoimmune diseases: myocarditis and thyroiditis. The people working at the moment in the laboratory, their expertise and research interests are listed below. For fellowship opportunities, please contact Dr. Rose, or Dr. Burek, or Dr. Caturegli.
 

Afanasyeva, Marina MD, MPH,PhD
I received an M.D. degree from the Minsk State Medical Institute, Minsk, Belarus in 1993. After a 1-year internship in internal medicine, I came to Baltimore where I first obtained an M.P.H. degree from the School of Hygiene and Public Health of the Johns Hopkins University in 1996. The same year, I was enrolled into the Ph.D. degree program at the W. Harry Feinstone Department of Molecular Microbiology and Immunology at the Johns Hopkins University. I am currently working on my Ph.D. thesis under Dr. Rose's supervision. My main interest is the role of cytokines in the development of autoimmune myocarditis. Using a model of cardiac myosin-induced autoimmune myocarditis, I have found that a Th2 cytokine, IL-4, promotes myocarditis in A/J mice. Now, my research is focused on a Th1 cytokine, IFN-g. IFN-g-deficient mice develop very severe myocarditis with subsequent development of cardiomyopathy and impairment of cardiac function. Intrigued by this finding, I started looking for the mechanisms of a disease-limiting action of IFN-g.

Barin, Jobert

Fairweather, Delisa PhD
I am interested in the pathogenesis of the myocarditis induced by Coxsackie virus B3. In particular I focus on the role of the innate immune response to the virus in the development of autoimmune disease.

Kimura, Hiroaki PhD

Kimura, Miho

Sharma, Rajni PhD
I obtained a PhD degree from Meerut University, India in 1994 and I developed my interest in autoimmunity since then. I joined this laboratory in 2000 as postdoctoral fellow, to study the role of iodine in the induction of autoimmune thyroiditis. I use the mouse NOD H2h4, which has lost the spontaneous development of diabetes as the parental strain NOD, and has acquired the spontaneous development of thyroiditis. I am looking at the control that NK T cells exert on the development of thyroiditis.

 
Research Laboratory
Our clinical laboratory specializes in the detection of autoantibodies, using immunofluorescence, hemagglutination or ELISA techniques. At the moment the menu offers 13 autoantibody tests, which are performed by Monica Talor and Anita Mallappa.

Anita Mallappa

Monica Talor

 

    Markers of autoimmune endocrinopathies
  1. Thyroglobulin antibodies (hemagglutination)
  2. Microsomal (TPO) antibodies (hemagglutination)
  3. Adrenal antibodies (indirect immunofluorescence)

Autoantibodies to the two major thyroid antigens, thyroglobulin and thyroid peroxidase (TPO) are found in autoimmune thyroid conditions. A subset of patients may also develop antibodies to gastric parietal cells or to the adrenal gland. These latter antibodies are also found by themselves in other autoimmune-associated gastritic disorders or in Addison's disease, respectively.
    Markers of autoimmune cardiovascular disorders
  1. Cardiac antibodies (indirect immunofluorescence)
  2. ANCA (indirect immunofluorescence)
  3. PR3 (ELISA)
  4. MPO (ELISA)
Many types of necrotizing vasculitis disorders involving small to medium size vessels have been associated with antibodies to cytoplasmic antigens of neutrophils. Some of the disorders are Wegener's granulomatosis, crescentic necrotizing glomerulonephritis, and microscopic polyangiitis. Two patterns are seen by indirect immunofluorescence: cytoplasmic (c-ANCA) or perinuclear (p-ANCA). The major antigen for c-ANCA is a serine protease 3 (PR3), while the major antigen for p-ANCA is myeloperoxidase (MPO).
    Markers of autoimmune gastro-intestinal disorders
  1. Smooth muscle antibodies (indirect immunofluorescence)
  2. Mitochondrial antibodies (indirect immunofluorescence)
  3. IgA endomysial antibodies (indirect immunofluorescence)
  4. IgA reticulin antibodies (indirect immunofluorescence)
  5. Tissue transglutaminase (ELISA)
  6. Gastric parietal cell antibodies (indirect immunofluorescence)

Several autoantibodies are associated with different forms of autoimmune intestinal disorders. Smooth muscle antibodies are found in chronic autoimmune hepatitis, while mitochondrial antibodies are associated with primary biliary cirrhosis. An atypical p-ANCA is seen in inflammatory bowel disease or chronic sclerosing cholangitis. Celiac disease has a high association with an IgA antibody to endomysium or, less frequently, to reticulin.

 



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